2nd February 2018,
Joanne Hewitt, David Harte, Michelle Sutherland, Dawn Croucher, Lindsey Fouche, Peter Flanagan, Deborah Williamson
Hepatitis E virus (HEV) infection is a common cause of acute hepatitis in developing countries, where faecal-oral transmission via faecally contaminated water is the most common transmission route.1 Hepatitis E…
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Hepatitis E virus (HEV) can be transmitted by blood transfusion. This study measured the presence of HEV antibodies (evidence of past or present infection) in New Zealand blood donors. This was determined to be 8.1 to 9.7% using two different antibody tests. The rate in New Zealand is similar to that reported in other developed countries. HEV was not found in blood samples from 5,000 donations using a molecular test to detect viral nucleic acid. This indicated no evidence of current HEV infection. This study is the largest to date to assess past and current HEV infection in New Zealand blood donors.
Blood transfusion is one route of transmission of hepatitis E virus (HEV). The aim of this study was to assess both the prevalence of HEV antibodies and HEV infection in New Zealand blood donors.
To determine HEV seroprevalence, donor plasma samples (n=1,013) were tested for HEV antibodies using two commercially available ELISA kits, the Wantai HEV IgG ELISA and the MP Diagnostics HEV ELISA 4.0. To assess the prevalence of HEV infection, pooled plasma samples from individual plasma donors (n=5,000) were tested for HEV RNA using RT-qPCR. Samples that tested HEV antibody positive or gave an equivocal result with either ELISA were also tested for HEV RNA.
The HEV seroprevalence in New Zealand blood donors was 9.7% using the Wantai HEV IgG ELISA and 8.1% using the MP Diagnostics HEV ELISA 4.0. The presence of HEV antibodies was significantly and positively correlated with increasing donor age. HEV RNA was not detected in any of the samples tested, indicating no evidence of current infection.
This study, the largest to date to assess HEV seroprevalence in New Zealand, provides valuable baseline information on HEV seroprevalence and infection in New Zealand blood donors. The seroprevalence rate in New Zealand is similar to that reported in other developed countries.
- Perez-Gracia MT, Garcia M, Suay B, et al. Current knowledge on hepatitis E. Eur J Clin Transl Hepatol. 2015; 3:117–26.
- Faber MS, Wenzel JJ, Jilg W, et al. Hepatitis E virus seroprevalence among adults, Germany. Emerg Infect Dis. 2012; 18:1654–7.
- Park WJ, Park BJ, Ahn HS, et al. Hepatitis E virus as an emerging zoonotic pathogen. J Vet Med Sci 2016; 17:1–11.
- Sridhar S, Lau SKP, Woo PCY. Hepatitis E: A disease of reemerging importance. J Formos Med Assoc. 2015; 114:681–90.
- Tei S, Kitajima N, Takahashi K, et al. Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet. 2003; 362:371–3.
- Matsubayashi K, Kang J-H, Sakata H, et al. A case of transfusion-transmitted hepatitis E caused by blood from a donor infected with hepatitis E virus via zoonotic food-borne route. Transfusion. 2008; 48:1368–75.
- Dreier J, Juhl D. Autochthonous hepatitis E virus infections: A new transfusion-associated risk? Transfus Med Hemother. 2014; 41:29–39.
- Goel A, Aggarwal R. Advances in hepatitis E - II: Epidemiology, clinical manifestations, treatment and prevention. Expert Rev Gastroenterol Hepatol. 2016; 10:1065–74.
- Cleland A, Smith L, Crossan C, et al. Hepatitis E virus in Scottish blood donors. Vox Sang. 2013; 105:283–9.
- Slot E, Hogema BM, Riezebos-Brilman A, et al. Silent hepatitis E virus infection in Dutch blood donors, 2011 to 2012. Euro Surveill. 2013; 18:20550.
- Lucarelli C, Spada E, Taliani G, et al. High prevalence of anti-hepatitis E virus antibodies among blood donors in central Italy, February to March 2014. Euro Surveill. 2016; 21:6–15.
- Stramer SL, Moritz ED, Foster GA, et al. Hepatitis E virus: Seroprevalence and frequency of viral RNA detection among US blood donors. Transfusion. 2016; 56:481–8.
- Shrestha AC, Flower RLP, Seed CR, et al. Hepatitis E virus RNA in Australian blood donations. Transfusion. 2016; 56:3086–93.
- Ijaz S, Szypulska R, Tettmar KI, et al. Detection of hepatitis E virus RNA in plasma mini-pools from blood donors in England. Vox Sang. 2012; 102:272.
- Domanovic D, Tedder R, Blumel J, et al. Hepatitis E and blood donation safety in selected European countries: a shift to screening? Euro Surveill. 2017; 22.
- Dalton HR, Fellows HJ, Gane EJ, et al. Hepatitis E in New Zealand. J Gastroenterol Hepatol 2007; 22:1236–40.
- Wenzel JJ, Preiss J, Schemmerer M, et al. Test performance characteristics of anti-HEV IgG assays strongly influence hepatitis E seroprevalence estimates. J Infect Dis. 2013; 207:497–500.
- R Development Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria., 2014.
- Beale MA, Tettmar K, Szypulska R, et al. Is there evidence of recent hepatitis E virus infection in English and North Welsh blood donors? Vox Sang. 2011; 100:340–2.
- O’Riordan J, Boland F, Williams P, et al. Hepatitis E virus infection in the Irish blood donor population. Transfusion. 2016; 56:2868–76.
- Shrestha AC, Seed CR, Flower RL, et al. Hepatitis E virus and implications for blood supply safety, Australia. Emerg Infect Dis. 2014; 20:1940–2.
- Fischer C, Hofmann M, Danzer M, et al. Seroprevalence and incidence of hepatitis E in blood donors in Upper Austria. PLoS ONE. 2015; 10:e0119576.
- Holm DK, Moessner BK, Engle RE, et al. Declining prevalence of hepatitis E antibodies among Danish blood donors. Transfusion. 2015; 55:1662–7.
- Lange H, Overbo J, Borgen K, et al. Hepatitis E in Norway: Seroprevalence in humans and swine. Epidemiol Infect. 2017; 145:181–6.
- Mansuy JM, Gallian P, Dimeglio C, et al. A nationwide survey of hepatitis E viral infection in French blood donors. Hepatology. 2016; 63:1145–54.
- Ma L, Sun P, Lin F, et al. Prevalence of hepatitis E virus in Chinese blood donors. J Int Med Res. 2015; 43:257–62.
- Bendall R, Ellis V, Ijaz S, et al. A comparison of two commercially available anti-HEV IgG kits and a re-evaluation of anti-HEV IgG seroprevalence data in developed countries. J Med Virol. 2010; 82:799–805.
- Shrestha AC, Flower RL, Seed CR, et al. A comparative study of assay performance of commercial hepatitis E virus enzyme-linked immunosorbent assay kits in Australian blood donor samples. Blood Transfus. 2016; 2016:9647675.
- Hartl J, Otto B, Madden RG, et al. Hepatitis E seroprevalence in Europe: A meta-analysis. Viruses. 2016; 8.
- Kmush BL, Labrique AB, Dalton HR, et al. Two generations of “gold standards”: The impact of a decade in hepatitis E virus testing innovation on population seroprevalence. Am J Trop Med Hyg 2015; 93:714–7.
- Petrik J, Lozano M, Seed CR, et al. Hepatitis E. Vox Sang. 2016; 110:93–103.
- Xu C, Wang RY, Schechterly CA, et al. An assessment of hepatitis E virus (HEV) in US blood donors and recipients. Transfusion. 2013; 53:2505–11.
- Hogema BM, Molier M, Slot E, et al. Past and present of hepatitis E in the Netherlands. Transfusion. 2014; 54:3092–6.
- Zhuang W, Ding X, Lyu C, et al. Hepatitis E virus seroprevalence among blood donors in Jiangsu Province, East China. Int J Infect Dis 2014; 26:9–11.
- Berto A, Martelli F, Grierson S, et al. Hepatitis E virus in pork food chain, United Kingdom, 2009–2010. Emerg Infect Dis. 2012; 18:1358–60.
- Feagins AR, Opriessnig T, Guenette DK, et al. Detection and characterization of infectious Hepatitis E virus from commercial pig livers sold in local grocery stores in the USA. J Gen Virol. 2007; 88:912–7.
- Garkavenko O, Obriadina A, Meng J, et al. Detection and characterisation of swine hepatitis E virus in New Zealand. J Med Virol. 2001; 65:525–9.
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